Top Active Pre-Seed AI Investors & VCs Funding Startups in the US in 2025

Last update to database: October 13, 2025

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Leading Early-Stage Investors & Venture Capital Firms Funding Pre-Seed AI Startups Across the US

Artificial intelligence continues to attract early-stage venture capital in 2025, as investors seek to fund innovation from the very first round. The top active pre-seed investors and VCs funding AI startups in the US are backing breakthroughs in machine learning, generative AI, computer vision, and automation technologies.


These investors provide the earliest institutional capital - helping founders validate ideas, build prototypes, and prepare for seed or Series A growth. Many are micro-VCs, accelerators, and angel syndicates headquartered in tech hubs like San Francisco, New York, Austin, and Boston, while others are global funds investing in American AI innovation.


Our database highlights investors currently funding and supporting pre-seed AI startups across the United States. Updated weekly, it gives founders, analysts, and business development teams real-time visibility into who’s actively investing in early-stage artificial intelligence companies nationwide.

Comprehensive Database of Active Pre-Seed AI Investors & VCs Funding US Startups – Updated Weekly

This data is delayed by 4 weeks. Subscribe for instant access to real-time funding rounds and contact data.

Investor Name
Industry Focus Recent Investments Recent Investment Date Check Size Stage Focus Geography Focus Company Headquarters Firm Type Portfolio Size Portfolio Highlights Co-Investor Network
Anthropic Artificial Intelligence, Information Technology, SaaS & Software, Data & Analytics, Business & Professional Services Subscribe to Unlock Subscribe to Unlock October, 2025 $1M-$10M Series C, Series D United States San Francisco, California, United States Other 10 Subscribe to Unlock Subscribe to Unlock ICONIQ, Lightspeed Venture Partners, Fidelity Management & Research Company, Altimeter, Baillie Gifford
Orascom Manufacturing, Construction, Infrastructure, Industrial, Facility Management Subscribe to Unlock Subscribe to Unlock September, 2025 $20M+ Series B, Series C, Series D Middle East, Africa, United States Cairo, Egypt Corporate VC 15 Subscribe to Unlock Subscribe to Unlock Unknown
JME Ventures SaaS & Software, Information Technology, Financial Services & FinTech, EdTech and Training, Apps & Platforms Subscribe to Unlock Subscribe to Unlock September, 2025 $100k-$1M Seed, Series A Europe, United States, Spain Madrid, Community of Madrid, Spain Venture Capital 70 Subscribe to Unlock Subscribe to Unlock Top global investors and operators (not specifically named)
Augur Vc Artificial Intelligence, Energy, SaaS & Software Subscribe to Unlock Subscribe to Unlock September, 2025 $100k-$250k Pre-Seed, Seed North America Calgary, Alberta, Canada Venture Capital 11 Subscribe to Unlock Subscribe to Unlock Unknown
Lombardstreet Ventures Business & Professional Services, SaaS & Software, Developer Tools, Artificial Intelligence, Infrastructure Software Subscribe to Unlock Subscribe to Unlock September, 2025 $100k-$250k Pre-Seed, Seed United States, Silicon Valley Menlo Park, California, United States Venture Capital 90 Subscribe to Unlock Subscribe to Unlock Unknown
Y Combinator Apps & Platforms, SaaS & Software, Artificial Intelligence, Biotechnology, EdTech and Training Subscribe to Unlock Subscribe to Unlock October, 2025 $500k-$1M Pre-Seed, Seed, Series A, Series B United States, North America United States Accelerator 5000 Subscribe to Unlock Subscribe to Unlock Top startup investors (network introduced by YC)
VentureFriends SaaS & Software, Marketplaces, Financial Services & FinTech, E-Commerce & Retail, Business & Professional Services Subscribe to Unlock Subscribe to Unlock September, 2025 $500k-$1M Pre-Seed, Seed Europe, Middle East Athens, Greece Venture Capital 100 Subscribe to Unlock Subscribe to Unlock Accel, Index Ventures, Balderton Capital
Khosla Ventures Artificial Intelligence, Health Care & Wellness, GreenTech & Sustainability, SaaS & Software, Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock October, 2025 $1M–$10M Pre-seed, Seed, Series A, Series B, Growth United States, Global Menlo Park, California, United States Venture Capital 100 Subscribe to Unlock Subscribe to Unlock Unknown
General Catalyst Artificial Intelligence, Health Care & Wellness, Financial Services & FinTech, SaaS & Software, Enterprise Subscribe to Unlock Subscribe to Unlock October, 2025 $1M–$10M Seed, Series A, Series B, Growth North America, Europe, Asia Cambridge, Massachusetts, United States Venture Capital 800 Subscribe to Unlock Subscribe to Unlock GC Famiglia (community of CEOs, founders, partners, policymakers)
A2D Ventures Artificial Intelligence, SaaS & Software, Financial Services & FinTech, Health Care & Wellness, E-Commerce & Retail Subscribe to Unlock Subscribe to Unlock September, 2025 $250k-$500k Pre-Seed, Seed, Series A Asia, Singapore, Thailand, Vietnam, Malaysia, Indonesia, Philippines Bangkok, Thailand Angel Investor 17 Subscribe to Unlock Subscribe to Unlock BEENEXT, East Ventures, Golden Gate Ventures, Wavemaker Partners
Team Ignite Ventures Financial Services & FinTech, Artificial Intelligence, SaaS & Software, Marketing, Advertising & Sales Subscribe to Unlock Subscribe to Unlock September, 2025 $100k-$250k Pre-Seed, Seed, Series A United States, Singapore, Nigeria, Canada, United Kingdom, Pakistan NorCal, California, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Y-Combinator, Andreessen Horowitz, Sequoia, Greylock, Techstars
CIV Advanced Materials, Artificial Intelligence, Energy, Logistics, Transportation Subscribe to Unlock Subscribe to Unlock September, 2025 $10M–$20M Pre-seed, Seed, Series A, Series B United States, California Venice, California, United States Venture Capital 7 Subscribe to Unlock Subscribe to Unlock Zero Infinity Partners
Buff Gold Ventures Deep Tech (Technology not a formal option) - breakdown: Electronics & Devices, Environmental, Health Care & Wellness, Artificial Intelligence, Biotechnology Subscribe to Unlock Subscribe to Unlock August, 2025 $100k-$250k Pre-Seed, Seed, Series A United States Boulder, Colorado, United States Venture Capital 4 Subscribe to Unlock Subscribe to Unlock Unknown
M25 Information Technology, SaaS & Software Subscribe to Unlock Subscribe to Unlock October, 2025 $100k-$250k Pre-Seed, Seed United States, Midwest, including western Pennsylvania and Kentucky Chicago, Illinois, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Unknown
Sputnik ATX VC SaaS & Software, Information Technology, Business & Professional Services, Sales Training, Startups Subscribe to Unlock Subscribe to Unlock August, 2025 $100k-$250k Pre-Seed United States, Austin, TX Austin, Texas, United States Accelerator 20 Subscribe to Unlock Subscribe to Unlock Unknown

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