Top Active Pre-Seed AI Investors & VCs Funding Startups in the US in 2025
Last update to database: October 13, 2025
Leading Early-Stage Investors & Venture Capital Firms Funding Pre-Seed AI Startups Across the US
Artificial intelligence continues to attract early-stage venture capital in 2025, as investors seek to fund innovation from the very first round. The top active pre-seed investors and VCs funding AI startups in the US are backing breakthroughs in machine learning, generative AI, computer vision, and automation technologies.
These investors provide the earliest institutional capital - helping founders validate ideas, build prototypes, and prepare for seed or Series A growth. Many are micro-VCs, accelerators, and angel syndicates headquartered in tech hubs like San Francisco, New York, Austin, and Boston, while others are global funds investing in American AI innovation.
Our database highlights investors currently funding and supporting pre-seed AI startups across the United States. Updated weekly, it gives founders, analysts, and business development teams real-time visibility into who’s actively investing in early-stage artificial intelligence companies nationwide.
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Top Active Pre-Seed AI Investors & VCs Funding Startups in the US in 2025
Last update to database: October 13, 2025
Subscribe to Unlock Active Startup Investors
Request AccessConnect with active investors funding startups in your region or industry. Subscribe to unlock full firm and contact data.
Leading Early-Stage Investors & Venture Capital Firms Funding Pre-Seed AI Startups Across the US
Artificial intelligence continues to attract early-stage venture capital in 2025, as investors seek to fund innovation from the very first round. The top active pre-seed investors and VCs funding AI startups in the US are backing breakthroughs in machine learning, generative AI, computer vision, and automation technologies.
These investors provide the earliest institutional capital - helping founders validate ideas, build prototypes, and prepare for seed or Series A growth. Many are micro-VCs, accelerators, and angel syndicates headquartered in tech hubs like San Francisco, New York, Austin, and Boston, while others are global funds investing in American AI innovation.
Our database highlights investors currently funding and supporting pre-seed AI startups across the United States. Updated weekly, it gives founders, analysts, and business development teams real-time visibility into who’s actively investing in early-stage artificial intelligence companies nationwide.
Comprehensive Database of Active Pre-Seed AI Investors & VCs Funding US Startups – Updated Weekly
This data is delayed by 4 weeks. Subscribe for instant access to real-time funding rounds and contact data.
Investor Name
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Industry Focus | Recent Investments | Recent Investment Date | Check Size | Stage Focus | Geography Focus | Company Headquarters | Firm Type | Portfolio Size | Portfolio Highlights | Co-Investor Network |
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Anthropic | Artificial Intelligence, Information Technology, SaaS & Software, Data & Analytics, Business & Professional Services | Subscribe to Unlock Subscribe to Unlock | October, 2025 | $1M-$10M | Series C, Series D | United States | San Francisco, California, United States | Other | 10 | Subscribe to Unlock Subscribe to Unlock | ICONIQ, Lightspeed Venture Partners, Fidelity Management & Research Company, Altimeter, Baillie Gifford |
Orascom | Manufacturing, Construction, Infrastructure, Industrial, Facility Management | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $20M+ | Series B, Series C, Series D | Middle East, Africa, United States | Cairo, Egypt | Corporate VC | 15 | Subscribe to Unlock Subscribe to Unlock | Unknown |
JME Ventures | SaaS & Software, Information Technology, Financial Services & FinTech, EdTech and Training, Apps & Platforms | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $100k-$1M | Seed, Series A | Europe, United States, Spain | Madrid, Community of Madrid, Spain | Venture Capital | 70 | Subscribe to Unlock Subscribe to Unlock | Top global investors and operators (not specifically named) |
Augur Vc | Artificial Intelligence, Energy, SaaS & Software | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $100k-$250k | Pre-Seed, Seed | North America | Calgary, Alberta, Canada | Venture Capital | 11 | Subscribe to Unlock Subscribe to Unlock | Unknown |
Lombardstreet Ventures | Business & Professional Services, SaaS & Software, Developer Tools, Artificial Intelligence, Infrastructure Software | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $100k-$250k | Pre-Seed, Seed | United States, Silicon Valley | Menlo Park, California, United States | Venture Capital | 90 | Subscribe to Unlock Subscribe to Unlock | Unknown |
Y Combinator | Apps & Platforms, SaaS & Software, Artificial Intelligence, Biotechnology, EdTech and Training | Subscribe to Unlock Subscribe to Unlock | October, 2025 | $500k-$1M | Pre-Seed, Seed, Series A, Series B | United States, North America | United States | Accelerator | 5000 | Subscribe to Unlock Subscribe to Unlock | Top startup investors (network introduced by YC) |
VentureFriends | SaaS & Software, Marketplaces, Financial Services & FinTech, E-Commerce & Retail, Business & Professional Services | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $500k-$1M | Pre-Seed, Seed | Europe, Middle East | Athens, Greece | Venture Capital | 100 | Subscribe to Unlock Subscribe to Unlock | Accel, Index Ventures, Balderton Capital |
Khosla Ventures | Artificial Intelligence, Health Care & Wellness, GreenTech & Sustainability, SaaS & Software, Financial Services & FinTech | Subscribe to Unlock Subscribe to Unlock | October, 2025 | $1M–$10M | Pre-seed, Seed, Series A, Series B, Growth | United States, Global | Menlo Park, California, United States | Venture Capital | 100 | Subscribe to Unlock Subscribe to Unlock | Unknown |
General Catalyst | Artificial Intelligence, Health Care & Wellness, Financial Services & FinTech, SaaS & Software, Enterprise | Subscribe to Unlock Subscribe to Unlock | October, 2025 | $1M–$10M | Seed, Series A, Series B, Growth | North America, Europe, Asia | Cambridge, Massachusetts, United States | Venture Capital | 800 | Subscribe to Unlock Subscribe to Unlock | GC Famiglia (community of CEOs, founders, partners, policymakers) |
A2D Ventures | Artificial Intelligence, SaaS & Software, Financial Services & FinTech, Health Care & Wellness, E-Commerce & Retail | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $250k-$500k | Pre-Seed, Seed, Series A | Asia, Singapore, Thailand, Vietnam, Malaysia, Indonesia, Philippines | Bangkok, Thailand | Angel Investor | 17 | Subscribe to Unlock Subscribe to Unlock | BEENEXT, East Ventures, Golden Gate Ventures, Wavemaker Partners |
Team Ignite Ventures | Financial Services & FinTech, Artificial Intelligence, SaaS & Software, Marketing, Advertising & Sales | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $100k-$250k | Pre-Seed, Seed, Series A | United States, Singapore, Nigeria, Canada, United Kingdom, Pakistan | NorCal, California, United States | Venture Capital | 40 | Subscribe to Unlock Subscribe to Unlock | Y-Combinator, Andreessen Horowitz, Sequoia, Greylock, Techstars |
CIV | Advanced Materials, Artificial Intelligence, Energy, Logistics, Transportation | Subscribe to Unlock Subscribe to Unlock | September, 2025 | $10M–$20M | Pre-seed, Seed, Series A, Series B | United States, California | Venice, California, United States | Venture Capital | 7 | Subscribe to Unlock Subscribe to Unlock | Zero Infinity Partners |
Buff Gold Ventures | Deep Tech (Technology not a formal option) - breakdown: Electronics & Devices, Environmental, Health Care & Wellness, Artificial Intelligence, Biotechnology | Subscribe to Unlock Subscribe to Unlock | August, 2025 | $100k-$250k | Pre-Seed, Seed, Series A | United States | Boulder, Colorado, United States | Venture Capital | 4 | Subscribe to Unlock Subscribe to Unlock | Unknown |
M25 | Information Technology, SaaS & Software | Subscribe to Unlock Subscribe to Unlock | October, 2025 | $100k-$250k | Pre-Seed, Seed | United States, Midwest, including western Pennsylvania and Kentucky | Chicago, Illinois, United States | Venture Capital | 40 | Subscribe to Unlock Subscribe to Unlock | Unknown |
Sputnik ATX VC | SaaS & Software, Information Technology, Business & Professional Services, Sales Training, Startups | Subscribe to Unlock Subscribe to Unlock | August, 2025 | $100k-$250k | Pre-Seed | United States, Austin, TX | Austin, Texas, United States | Accelerator | 20 | Subscribe to Unlock Subscribe to Unlock | Unknown |
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