Top Active Pre-Seed AI Investors & VCs Funding Startups in the US in 2025

Last update to database: May 25, 2026

Leading Early-Stage Investors & Venture Capital Firms Funding Pre-Seed AI Startups Across the US

Artificial intelligence continues to attract early-stage venture capital in 2025, as investors seek to fund innovation from the very first round. The top active pre-seed investors and VCs funding AI startups in the US are backing breakthroughs in machine learning, generative AI, computer vision, and automation technologies.


These investors provide the earliest institutional capital - helping founders validate ideas, build prototypes, and prepare for seed or Series A growth. Many are micro-VCs, accelerators, and angel syndicates headquartered in tech hubs like San Francisco, New York, Austin, and Boston, while others are global funds investing in American AI innovation.


Our database highlights investors currently funding and supporting pre-seed AI startups across the United States. Updated weekly, it gives founders, analysts, and business development teams real-time visibility into who’s actively investing in early-stage artificial intelligence companies nationwide.

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Comprehensive Database of Active Pre-Seed AI Investors & VCs Funding US Startups – Updated Weekly

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Investor Name
Industry Focus Recent Investments Recent Investment Date Check Size Stage Focus Geography Focus Company Headquarters Firm Type Portfolio Size Portfolio Highlights Co-Investor Network
ShuckerVC SaaS & Software, Artificial Intelligence, Data & Analytics, Business & Professional Services, Information Technology Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Seed United States, Silicon Valley San Mateo, California, United States Venture Capital 3 Subscribe to Unlock Subscribe to Unlock Shop-Ware, PartsTech, BDev Ventures
Episode 1 Ventures SaaS & Software, Artificial Intelligence, Information Technology, Apps & Platforms Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Pre-Seed, Seed United Kingdom, Europe London, England and Wales Venture Capital 65 Subscribe to Unlock Subscribe to Unlock Unknown
All In Capital Apps & Platforms, Information Technology, SaaS & Software Subscribe to Unlock Subscribe to Unlock May, 2026 $250k-$500k Pre-Seed, Seed India Mumbai, India Venture Capital 150 Subscribe to Unlock Subscribe to Unlock Unknown
Norrsken Evolve GreenTech & Sustainability, Energy, Health Care & Wellness, Property & Real Estate, Food & Drink Subscribe to Unlock Subscribe to Unlock May, 2026 $250k-$500k Pre-Seed, Seed Europe, United Kingdom Stockholm, Stockholm County, Sweden Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Unknown
Updata Partners SaaS & Software, Business & Professional Services, Information Technology, Data & Analytics, Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock April, 2026 $10M-$20M Series B, Series C, Series D United States Washington, District of Columbia, United States Private Equity 80 Subscribe to Unlock Subscribe to Unlock Unknown
Gaingels Technology, Business & Professional Services (B2B), Health Care & Wellness, Consumer Subscribe to Unlock Subscribe to Unlock May, 2026 $100k-$250k Seed, Series A, Series B, Series C, Series D United States, India, Global Burlington, Vermont, United States Venture Capital 2400 Subscribe to Unlock Subscribe to Unlock Select venture capital leads, diverse investors including LGBTQ community and allies
Focal Apps & Platforms, SaaS & Software, Information Technology, Artificial Intelligence, Data & Analytics Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Pre-Seed, Seed United States, Canada San Francisco, California, United States Venture Capital 30 Subscribe to Unlock Subscribe to Unlock Unknown
QED Investors Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock May, 2026 $1M-$10M Pre-Seed, Seed, Series A, Series B, Series C, Series D North America, Europe, Latin America, Asia, Africa Alexandria, Virginia, United States Venture Capital 225 Subscribe to Unlock Subscribe to Unlock Unknown
Dorm Room Fund Apps & Platforms, SaaS & Software, EdTech and Training, Business & Professional Services, Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock April, 2026 $50k-$100k Angel, Pre-Seed, Seed United States Cambridge, Massachusetts, United States Venture Capital 300 Subscribe to Unlock Subscribe to Unlock Unknown
Madrona Artificial Intelligence, SaaS & Software, Health Care & Wellness, Apps & Platforms, Data & Analytics Subscribe to Unlock Subscribe to Unlock April, 2026 $1M-$10M Seed, Series A, Series B, Series C United States, Seattle, Silicon Valley Seattle, Washington, United States Venture Capital 90 Subscribe to Unlock Subscribe to Unlock Andreessen Horowitz, First Round Capital, Floodgate, BAMCAP
Maple VC Artificial Intelligence, Blockchain & Cryptocurrency, SaaS & Software, Fashion & Apparel, Gaming Subscribe to Unlock Subscribe to Unlock April, 2026 $250k-$500k Pre-Seed, Seed, Series A, Series B Canada San Francisco, California, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Bain Capital Ventures, Cherubic Ventures, Fusion Fund, Altos Ventures, Haystack
Impellent Ventures SaaS & Software, Financial Services & FinTech, Healthcare & Wellness, Artificial Intelligence, Marketing, Advertising & Sales Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Pre-Seed, Seed, Series A United States Rochester, New York, United States Venture Capital 14 Subscribe to Unlock Subscribe to Unlock NextView Ventures, Hubspot Ventures, Accomplice, BMC, Asymmetric Capital Partners
Underscore VC SaaS & Software, Artificial Intelligence, Financial Services & FinTech, Business & Professional Services, EdTech and Training Subscribe to Unlock Subscribe to Unlock April, 2026 $100k-$250k Pre-Seed, Seed United States, Europe Boston, Massachusetts, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Unknown
Ewor EdTech and Training, Venture Capital Subscribe to Unlock Subscribe to Unlock May, 2026 $500k-$1M Pre-Seed, Seed Europe, virtual-first globally Berlin, Berlin, Germany Venture Capital 70 Subscribe to Unlock Subscribe to Unlock FJ Labs
Y Combinator Apps & Platforms, SaaS & Software, Artificial Intelligence, Biotechnology, EdTech and Training Subscribe to Unlock Subscribe to Unlock May, 2026 $500k-$1M Pre-Seed, Seed, Series A, Series B United States, North America United States Accelerator 5000 Subscribe to Unlock Subscribe to Unlock Top startup investors (network introduced by YC)
Antler SaaS & Software, Artificial Intelligence, Financial Services & FinTech, EdTech and Training, Health Care & Wellness Subscribe to Unlock Subscribe to Unlock May, 2026 $100k-$250k Angel, Pre-Seed, Seed, Series A, Series B, Series C North America, Europe, Asia, Middle East, Africa, Australia Singapore, Singapore Venture Capital 600 Subscribe to Unlock Subscribe to Unlock Sequoia Capital, Accel, Lightspeed Venture Partners, Craft Ventures, 500 Startups
AZ Venture Capital Enterprise Applications, Financial Services & FinTech, SaaS & Software, Information Technology, High Tech Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Seed, Series A United States, Singapore Phoenix, Arizona, United States Venture Capital 11 Subscribe to Unlock Subscribe to Unlock LiveOak Venture Partners, Brookstone Venture Capital, Next Coast Ventures
NFX Biotechnology, Gaming, Blockchain & Cryptocurrency, Deeptech, Financial Services & FinTech, Marketplaces (Apps & Platforms) Subscribe to Unlock Subscribe to Unlock May, 2026 $100k-$10M Pre-Seed, Seed United States, Israel, Latin America San Francisco, California, United States Venture Capital 120 Subscribe to Unlock Subscribe to Unlock Andreessen Horowitz, Greenspring Associates, Tiger Global Management, Y Combinator, FJ Labs
VYCAP Consumer, Enterprise Applications, Financial Services & FinTech, Blockchain & Cryptocurrency, SaaS & Software Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Seed, Series A, Series B, Series C, Series D United States, India Enschede, The Netherlands Venture Capital 54 Subscribe to Unlock Subscribe to Unlock Social Capital, Sequoia Capital, Founders Fund, Andreessen Horowitz
Gener8tor SaaS & Software, Health Care & Wellness, Design & Innovation, Education & Training, Creative Arts Subscribe to Unlock Subscribe to Unlock May, 2026 $100k-$250k Pre-Seed, Seed United States Madison, Wisconsin, United States Accelerator 1500 Subscribe to Unlock Subscribe to Unlock Boyd Street Ventures, Bronze Valley, ND Growth Fund, Serra Ventures, US Venture Partners
Emmeline Ventures Health Care & Wellness, Financial Services & FinTech, GreenTech & Sustainability, SaaS & Software, Apps & Platforms Subscribe to Unlock Subscribe to Unlock April, 2026 $50k-$500k Pre-Seed, Seed, Series A, Series B United States Los Angeles, California, United States Venture Capital 27 Subscribe to Unlock Subscribe to Unlock Unknown
Georgia Cleantech Innovation Hub Energy, GreenTech & Sustainability, Natural Resources, Manufacturing, Science & Engineering Subscribe to Unlock Subscribe to Unlock March, 2026 $100k-$250k Pre-Seed, Seed United States, Georgia Atlanta, Georgia, United States Accelerator 25 Subscribe to Unlock Subscribe to Unlock gener8tor, Cox Enterprises, Alabama Futures Fund
Tetrad VC B2B SaaS, Artificial Intelligence, Health Care & Wellness, Financial Services & FinTech, SaaS & Software Subscribe to Unlock Subscribe to Unlock March, 2026 $100k-$1M Angel, Pre-Seed, Seed United States, Europe, United Kingdom, Latin America, Middle East and North Africa Nicosia, Cyprus Venture Capital 30 Subscribe to Unlock Subscribe to Unlock Unknown
1984 Ventures Artificial Intelligence, SaaS & Software, Marketplaces, Health Care & Wellness, Robotics Subscribe to Unlock Subscribe to Unlock March, 2026 $500k-$1M Seed United States, San Francisco San Francisco, California, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Sequoia, General Catalyst, NFX, Index Ventures, Founders Fund
Pioneer Square Labs SaaS & Software, Artificial Intelligence, Financial Services & FinTech, Business & Professional Services, Enterprise Applications Subscribe to Unlock Subscribe to Unlock May, 2026 $1M-$10M Seed, Series A, Series B United States, Mexico Seattle, Washington, United States Venture Capital 52 Subscribe to Unlock Subscribe to Unlock ASCEND, Foundry, Trilogy Equity Partners, Insight Partners, Madrona, Clocktower Technology Ventures
Anterra Capital AgriTech & Farming, Biotechnology, Data & Analytics, SaaS & Software, Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Seed, Series A North America, Europe Boston, Massachusetts, Netherlands Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Rabobank, Eight Roads (Fidelity International), Novo Holdings, Tattarang
Offline Ventures Artificial Intelligence, Biotechnology, SaaS & Software, Health Care & Wellness, Design & Innovation Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Pre-Seed, Seed, Series A, Series C United States Mill Valley, California, United States Venture Capital 56 Subscribe to Unlock Subscribe to Unlock Andreessen Horowitz, Greenoaks Capital, Liquid 2 Ventures, D.E. Shaw, Eclipse Ventures
Andreessen Horowitz Artificial Intelligence, Biotechnology, Blockchain & Cryptocurrency, Financial Services & FinTech, Gaming Subscribe to Unlock Subscribe to Unlock May, 2026 $500k–$1M Pre-seed, Seed, Series A, Series B, Growth, Late-stage North America, United States Menlo Park, California, United States Venture Capital 1000 Subscribe to Unlock Subscribe to Unlock Thrive Capital, General Catalyst, Alumni Ventures, Sequoia Capital, Accel
Coinbase Blockchain & Cryptocurrency, Financial Services & FinTech, Apps & Platforms, Data & Analytics, SaaS & Software Subscribe to Unlock Subscribe to Unlock May, 2026 $1M-$10M Seed, Series A, Series B, Series C, Series D United States San Francisco, California, United States Corporate VC 461 Subscribe to Unlock Subscribe to Unlock Pantera Capital, Andreessen Horowitz, Polychain Capital, Dragonfly, Alameda Research
Link Ventures Artificial Intelligence, Financial Services & FinTech, Health Care & Wellness, E-Commerce & Retail, Cyber Security & Privacy Subscribe to Unlock Subscribe to Unlock May, 2026 $500k-$1M Pre-Seed, Seed, Series A United States, North America, Global Cambridge, Massachusetts, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock Unknown
Emergent Ventures Artificial Intelligence, SaaS & Software, Data & Analytics, Information Technology, Cloud & Data Infrastructure Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Pre-Seed, Seed, Series A United States San Mateo, California, United States Venture Capital 50 Subscribe to Unlock Subscribe to Unlock Lightspeed, Accel, ZoomInfo, Nutanix, Databricks
Fabric Ventures Artificial Intelligence, Blockchain & Cryptocurrency, Gaming, Financial Services & FinTech, Infrastructure Subscribe to Unlock Subscribe to Unlock May, 2026 $100k-$250k Pre-Seed, Seed, Series A, Series B Europe, United Kingdom, Germany, Switzerland, France, Poland, Australia London, United Kingdom Venture Capital 500 Subscribe to Unlock Subscribe to Unlock Unknown
Nadia Partners SaaS & Software, Artificial Intelligence, Energy, Financial Services & FinTech, Blockchain & Cryptocurrency Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Series A United States New York, New York, United States Venture Capital 6 Subscribe to Unlock Subscribe to Unlock Vestigo Ventures
Forum Ventures Business & Professional Services, SaaS & Software, Artificial Intelligence, Financial Services & FinTech, Apps & Platforms Subscribe to Unlock Subscribe to Unlock February, 2026 $100k-$250k Pre-Seed, Seed United States, Canada New York, New York, United States Venture Capital 430 Subscribe to Unlock Subscribe to Unlock NEA, Andreessen Horowitz, Uncork Capital, 8VC, Founders Fund
Informed Ventures Artificial Intelligence, Digital Health, SaaS & Software, Health Care & Wellness, Information Technology Subscribe to Unlock Subscribe to Unlock February, 2026 $500k-$1M Pre-Seed, Seed, Series A United States Menlo Park, California, United States Venture Capital 40 Subscribe to Unlock Subscribe to Unlock GSR Ventures, Data Collective DCVC, Lux Capital, First Round Capital, Sequoia Capital
South Loop Ventures Artificial Intelligence, Health Care & Wellness, Energy, SaaS & Software, Space & Climate Subscribe to Unlock Subscribe to Unlock February, 2026 $250k-$500k Pre-Seed, Seed United States, Houston Houston, Texas, United States Venture Capital 13 Subscribe to Unlock Subscribe to Unlock Rice Management Company, Chevron Technology Ventures, Texas Capital Bank, The Great Commission Foundation of the Episcopal Diocese of Texas, Mercury Fund
Front Porch Venture Partners Business & Professional Services, Health Care & Wellness, SaaS & Software, Information Technology, Innovation & Design Subscribe to Unlock Subscribe to Unlock February, 2026 $250k-$500k Seed, Series A United States, North Carolina Raleigh, North Carolina, United States Venture Capital 28 Subscribe to Unlock Subscribe to Unlock Triangle Tweener Fund, 8IDEA Fund Partners, Bull City Venture Partners, Cofounders Capital, Techstars
Kibo Ventures Information Technology, SaaS & Software, Data & Analytics, Financial Services & FinTech, Artificial Intelligence Subscribe to Unlock Subscribe to Unlock February, 2026 $500k-$1M Seed, Series A, Series B Europe, United States Madrid, Spain Venture Capital 75 Subscribe to Unlock Subscribe to Unlock Unknown
Lightspeed Artificial Intelligence, Cyber Security & Privacy, Biotechnology, SaaS & Software, Financial Services & FinTech Subscribe to Unlock Subscribe to Unlock May, 2026 $1M-$10M Seed, Series A, Series B, Series C, Series D United States, Europe, India, Israel, Southeast Asia Menlo Park, California, United States Venture Capital 500 Subscribe to Unlock Subscribe to Unlock Sequoia, Andreessen Horowitz, Accel, Greylock, Benchmark
Kfund SaaS & Software, Financial Services & FinTech, Artificial Intelligence, Apps & Platforms, Business & Professional Services Subscribe to Unlock Subscribe to Unlock April, 2026 $100k-$10M Pre-Seed, Seed, Series A, Series B Spain, Latin America Madrid, Spain Venture Capital 50 Subscribe to Unlock Subscribe to Unlock Okta, TeamSystem, Aldara, Arengu, Billin
Flint Capital Financial Services & FinTech, Health Care & Wellness, SaaS & Software, Cyber Security & Privacy, Business & Professional Services Subscribe to Unlock Subscribe to Unlock May, 2026 $500k-$1M Seed, Series A, Series B United States, Israel, Europe Boston, Massachusetts, United States Venture Capital 45 Subscribe to Unlock Subscribe to Unlock Glilot Capital Partners, Entrée Capital, Insight Partners, RTP Global, Yossi Matias
Great North Ventures Artificial Intelligence, Financial Services & FinTech, SaaS & Software, Data & Analytics, Hardware Subscribe to Unlock Subscribe to Unlock May, 2026 $500k-$1M Pre-Seed, Seed, Series A United States, Canada Minneapolis, Minnesota, United States Venture Capital 45 Subscribe to Unlock Subscribe to Unlock Gener8tor, Dundee Venture Capital, Revolution, Ironspring Ventures, Cohen Circle
Airtree SaaS & Software, Information Technology, Artificial Intelligence, Financial Services & FinTech, EdTech and Training Subscribe to Unlock Subscribe to Unlock May, 2026 $250k-$500k Seed, Series A, Series B, Series C Australia, New Zealand Surry Hills, New South Wales, Australia Venture Capital 120 Subscribe to Unlock Subscribe to Unlock Skip Capital, Blackbird Ventures, Saniel Ventures, Athletic Ventures, OneVentures
Zeal Capital Partners Enterprise Applications, Health Care & Wellness, SaaS & Software, Artificial Intelligence, Vertical SaaS Subscribe to Unlock Subscribe to Unlock March, 2026 $1M-$10M Seed, Series A, Series B United States Washington, District of Columbia, United States Venture Capital 61 Subscribe to Unlock Subscribe to Unlock Basecamp Fund, Drive Capital, Alumni Ventures, Emerson Collective, Rethink Impact
Precursor Ventures SaaS & Software, Hardware, Business & Professional Services, Consumer, Enterprise Subscribe to Unlock Subscribe to Unlock March, 2026 $100k-$250k Pre-Seed, Seed North America, United States San Francisco, California, United States Venture Capital 400 Subscribe to Unlock Subscribe to Unlock Unknown
Human Ventures Health Care & Wellness, SaaS & Software, Financial Services & FinTech, Artificial Intelligence, Social & Lifestyle Networks Subscribe to Unlock Subscribe to Unlock December, 2025 $100k-$250k Pre-Seed, Seed, Series A United States, New York New York, New York, United States Venture Capital 50 Subscribe to Unlock Subscribe to Unlock Unknown
SNR Health Care & Wellness, Property & Real Estate, Health Care & Wellness Subscribe to Unlock Subscribe to Unlock December, 2025 $10M-$20M Series C India San Francisco, California, United States Venture Capital 4 Subscribe to Unlock Subscribe to Unlock Sankosh Agro Farms, Timesgroup, Chiranjeevi Konidala, Elem Investments, Knowledge Crystals
Forward Venture Capital E-Commerce & Retail, SaaS & Software, Data & Analytics, Marketing, Advertising & Sales, Payment Solutions Subscribe to Unlock Subscribe to Unlock December, 2025 $50k-$500k Pre-Seed, Seed, Series A North America Salt Lake City, Utah, United States Venture Capital 50 Subscribe to Unlock Subscribe to Unlock Unknown
Kalaari Capital SaaS & Software, Artificial Intelligence, E-Commerce & Retail, Deeptech, EdTech and Training Subscribe to Unlock Subscribe to Unlock April, 2026 $500k-$1M Seed, Series A India Bengaluru, Karnataka, India Venture Capital 240 Subscribe to Unlock Subscribe to Unlock Avaana, Peak XV, Big Capital, Rakuten Capital
RTP Angel Fund Miscellaneous Subscribe to Unlock Subscribe to Unlock November, 2025 $100k-$250k Pre-Seed, Seed, Series A United States, North Carolina, Research Triangle Park Research Triangle Park, North Carolina, United States Angel Investor 50 Subscribe to Unlock Subscribe to Unlock Unknown

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